This is completed downloadable of Test Bank for Molecular Biology, 5th Edition: Robert Weaver

Product Details:

ISBN-10 ‏ : ‎ 0073525324
ISBN-13 ‏ : ‎ 978-0073525327
Author: Robert Weaver

Molecular Biology, 5/e by Robert Weaver, is designed for an introductory course in molecular biology. Molecular Biology 5/e focuses on the fundamental concepts of molecular biology emphasizing experimentation. In particular author, Rob Weaver, focuses on the study of genes and their activities at the molecular level. Through the combination of excellent illustrations and clear, succinct writing students are presented fundamental molecular biology concepts.

Table of Content:

PART I Introduction
CHAPTER 1 A Brief History
1.1 Transmission Genetics
Mendel’s Laws of Inheritance
The Chromosome Theory of Inheritance
Genetic Recombination and Mapping
Physical Evidence for Recombination
1.2 Molecular Genetics
The Discovery of DNA
The Relationship Between Genes and Proteins
Activities of Genes
1.3 The Three Domains of Life
CHAPTER 2 The Molecular Nature of Genes
2.1 The Nature of Genetic Material
Transformation in Bacteria
The Chemical Nature of Polynucleotides
2.2 DNA Structure
Experimental Background
The Double Helix
2.3 Genes Made of RNA
2.4 Physical Chemistry of Nucleic Acids
A Variety of DNA Structures
DNAs of Various Sizes and Shapes
CHAPTER 3 An Introduction to Gene Function
3.1 Storing Information
Overview of Gene Expression
Protein Structure
Protein Function
Discovery of Messenger RNA
Transcription
Translation
3.2 Replication
3.3 Mutations
Sickle Cell Disease
PART II Methods of Molecular Biology
CHAPTER 4 Molecular Cloning Methods
4.1 Gene Cloning
The Role of Restriction Endonucleases
Vectors
Identifying a Specific Clone with a Specific Probe
cDNA Cloning
Rapid Amplification of cDNA Ends
4.2 The Polymerase Chain Reaction
Standard PCR
Box 4.1 Jurassic Park: More than a Fantasy?
Using Reverse Transcriptase PCR (RT-PCR) in cDNA Cloning
Real-Time PCR
4.3 Methods of Expressing Cloned Genes
Expression Vectors
Other Eukaryotic Vectors
Using the Ti Plasmid to Transfer Genes to Plants
CHAPTER 5 Molecular Tools for Studying Genes and Gene Activity
5.1 Molecular Separations
Gel Electrophoresis
Two-Dimensional Gel Electrophoresis
Ion-Exchange Chromatography
Gel Filtration Chromatography
Affinity Chromatography
5.2 Labeled Tracers
Autoradiography
Phosphorimaging
Liquid Scintillation Counting
Nonradioactive Tracers
5.3 Using Nucleic Acid Hybridization
Southern Blots: Identifying Specific DNA Fragments
DNA Fingerprinting and DNA Typing
Forensic Uses of DNA Fingerprinting and DNA Typing
In Situ Hybridization: Locating Genes in Chromosomes
Immunoblots (Western Blots)
5.4 DNA Sequencing and Physical Mapping
The Sanger Chain-Termination Sequencing Method
Automated DNA Sequencing
High-Throughput Sequencing
Restriction Mapping
5.5 Protein Engineering with Cloned Genes: Site-Directed Mutagenesis
5.6 Mapping and Quantifying Transcripts
Northern Blots
S1 Mapping
Primer Extension
Run-Off Transcription and G-Less Cassette Transcription
5.7 Measuring Transcription Rates in Vivo
Nuclear Run-On Transcription
Reporter Gene Transcription
Measuring Protein Accumulation in Vivo
5.8 Assaying DNA–Protein Interactions
Filter Binding
Gel Mobility Shift
DNase Footprinting
DMS Footprinting and Other Footprinting Methods
Chromatin Immunoprecipitation (ChIP)
5.9 Assaying Protein–Protein Interactions
5.10 Finding RNA Sequences That Interact with Other Molecules
SELEX
Functional SELEX
5.11 Knockouts and Transgenics
Knockout Mice
Transgenic Mice
PART III Transcription in Bacteria
CHAPTER 6 The Mechanism of Transcription in Bacteria
6.1 RNA Polymerase Structure
Sigma (σ) as a Specificity Factor
6.2 Promoters
Binding of RNA Polymerase to Promoters
Promoter Structure
6.3 Transcription Initiation
Sigma Stimulates Transcription Initiation
Reuse of σ
The Stochastic σ-Cycle Model
Local DNA Melting at the Promoter
Promoter Clearance
Structure and Function of σ
The Role of the α-Subunit in UP Element Recognition
6.4 Elongation
Core Polymerase Functions in Elongation
Structure of the Elongation Complex
6.5 Termination of Transcription
Rho-Independent Termination
Rho-Dependent Termination
CHAPTER 7 Operons: Fine Control of Bacterial Transcription
7.1 The lac Operon
Negative Control of the lac Operon
Discovery of the Operon
Repressor–Operator Interactions
The Mechanism of Repression
Positive Control of the lac Operon
The Mechanism of CAP Action
7.2 The ara Operon
The ara Operon Repression Loop
Evidence for the ara Operon Repression Loop
Autoregulation of araC
7.3 The trp Operon
Tryptophan’s Role in Negative Control of the trp Operon
Control of the trp Operon by Attenuation
Defeating Attenuation
7.4 Riboswitches
CHAPTER 8 Major Shifts in Bacterial Transcription
8.1 Sigma Factor Switching
Phage Infection
Sporulation
Genes with Multiple Promoters
Other σ Switches
Anti-σ-Factors
8.2 The RNA Polymerase Encoded in Phage T7
8.3 Infection of E. coli by Phage λ
Lytic Reproduction of Phage λ
Establishing Lysogeny
Autoregulation of the cI Gene During Lysogeny
Determining the Fate of a λ Infection: Lysis or Lysogeny
Lysogen Induction
CHAPTER 9 DNA–Protein Interactions in Bacteria
9.1 The λ Family of Repressors
Probing Binding Specificity by Site-Directed Mutagenesis
Box 9.1 X-Ray Crystallography
High-Resolution Analysis of λ Repressor–Operator Interactions
High-Resolution Analysis of Phage 434 Repressor–Operator Interactions
9.2 The trp Repressor
The Role of Tryptophan
9.3 General Considerations on Protein–DNA Interactions
Hydrogen Bonding Capabilities of the Four Different Base Pairs
The Importance of Multimeric DNA-Binding Proteins
9.4 DNA-Binding Proteins: Action at a Distance
The gal Operon
Duplicated λ Operators
Enhancers
PART IV Transcription in Eukaryotes
CHAPTER 10 Eukaryotic RNA Polymerases and Their Promoters
10.1 Multiple Forms of Eukaryotic RNA Polymerase
Separation of the Three Nuclear Polymerases
The Roles of the Three RNA Polymerases
RNA Polymerase Subunit Structures
10.2 Promoters
Class II Promoters
Class I Promoters
Class III Promoters
10.3 Enhancers and Silencers
Enhancers
Silencers
CHAPTER 11 General Transcription Factors in Eukaryotes
11.1 Class II Factors
The Class II Preinitiation Complex
Structure and Function of TFIID
Structure and Function of TFIIB
Structure and Function of TFIIH
The Mediator Complex and the RNA Polymerase II Holoenzyme
Elongation Factors
11.2 Class I Factors
The Core-Binding Factor
The UPE-Binding Factor
Structure and Function of SL1
11.3 Class III Factors
TFIIIA
TFIIIB and C
The Role of TBP
CHAPTER 12 Transcription Activators in Eukaryotes
12.1 Categories of Activators
DNA-Binding Domains
Transcription-Activating Domains
12.2 Structures of the DNA-Binding Motifs of Activators
Zinc Fingers
The GAL4 Protein
The Nuclear Receptors
Homeodomains
The bZIP and bHLH Domains
12.3 Independence of the Domains of Activators
12.4 Functions of Activators
Recruitment of TFIID
Recruitment of the Holoenzyme
12.5 Interaction Among Activators
Dimerization
Action at a Distance
Box 12.1 Genomic Imprinting
Transcription Factories
Complex Enhancers
Architectural Transcription Factors
Enhanceosomes
Insulators
12.6 Regulation of Transcription Factors
Coactivators
Activator Ubiquitylation
Activator Sumoylation
Activator Acetylation
Signal Transduction Pathways
CHAPTER 13 Chromatin Structure and Its Effects on Transcription
13.1 Chromatin Structure
Histones
Nucleosomes
The 30-nm Fiber
Higher-Order Chromatin Folding
13.3 Chromatin Structure and Gene Activity
The Effects of Histones on Transcription of Class II Genes
Nucleosome Positioning
Histone Acetylation
Histone Deacetylation
Chromatin Remodeling
Heterochromatin and Silencing
Nucleosomes and Transcription Elongation
PART V Post-Transcriptional Events
CHAPTER 14 RNA Processing I: Splicing
14.1 Genes in Pieces
Evidence for Split Genes
RNA Splicing
Splicing Signals
Effect of Splicing on Gene Expression
14.2 The Mechanism of Splicing of Nuclear mRNA Precursors
A Branched Intermediate
A Signal at the Branch
Spliceosomes
Spliceosome Assembly and Function
Commitment, Splice Site Selection, and Alternative Splicing
Control of Splicing
14.3 Self-Splicing RNAs
Group I Introns
Group II Introns
CHAPTER 15 RNA Processing II: Capping and Polyadenylation
15.1 Capping
Cap Structure
Cap Synthesis
Functions of Caps
15.2 Polyadenylation
Poly(A)
Functions of Poly(A)
Basic Mechanism of Polyadenylation
Polyadenylation Signals
Cleavage and Polyadenylation of a Pre-mRNA
Poly(A) Polymerase
Turnover of Poly(A)
15.3 Coordination of mRNA Processing Events
Binding of the CTD of Rpb1 to mRNA-Processing Proteins
Changes in Association of RNA-Processing Proteins with the CTD Correlate with Changes in CTD Phospho
A CTD Code?
Coupling Transcription Termination with mRNA 3′-End Processing
Mechanism of Termination
Role of Polyadenylation in mRNA Transport
CHAPTER 16 Other RNA Processing Events and Post-Transcriptional Control of Gene Expression
16.1 Ribosomal RNA Processing
Eukaryotic rRNA Processing
Bacterial rRNA Processing
16.2 Transfer RNA Processing
Cutting Apart Polycistronic Precursors
Forming Mature 5′-Ends
Forming Mature 3′-Ends
16.3 Trans-Splicing
The Mechanism of Trans-Splicing
16.4 RNA Editing
Mechanism of Editing
Editing by Nucleotide Deamination
16.5 Post-Transcriptional Control of Gene Expression: mRNA Stability
Casein mRNA Stability
Transferrin Receptor mRNA Stability
16.6 Post-Transcriptional Control of Gene Expression: RNA Interference
Mechanism of RNAi
Amplification of siRNA
Role of the RNAi Machinery in Heterochromatin Formation and Gene Silencing
16.7 Piwi-Interacting RNAs and Transposon Control
16.8 Post-Transcriptional Control of Gene Expression: MicroRNAs
Silencing of Translation by miRNAs
Stimulation of Translation by miRNAs
16.9 Translation Repression, mRNA Degradation, and P-Bodies
Processing Bodies
Degradation of mRNAs in P-Bodies
Relief of Repression in P-Bodies
Other Small RNAs
PART VI Translation
CHAPTER 17 The Mechanism of Translation I: Initiation
17.1 Initiation of Translation in Bacteria
tRNA Charging
Dissociation of Ribosomes
Formation of the 30S Initiation Complex
Formation of the 70S Initiation Complex
Summary of Initiation in Bacteria
17.2 Initiation in Eukaryotes
The Scanning Model of Initiation
Eukaryotic Initiation Factors
17.3 Control of Initiation
Bacterial Translational Control
Eukaryotic Translational Control
CHAPTER 18 The Mechanism of Translation II: Elongation and Termination
18.1 The Direction of Polypeptide Synthesis and of mRNA Translation
18.2 The Genetic Code
Nonoverlapping Codons
No Gaps in the Code
The Triplet Code
Breaking the Code
Unusual Base Pairs Between Codon and Anticodon
The (Almost) Universal Code
18.3 The Elongation Cycle
Overview of Elongation
A Three-Site Model of the Ribosome
Elongation Step 1: Binding an Aminoacyl-tRNA to the A Site of the Ribosome
Elongation Step 2: Peptide Bond Formation
Elongation Step 3: Translocation
G Proteins and Translation
The Structures of EF-Tu and EF-G
18.4 Termination
Termination Codons
Stop Codon Suppression
Release Factors
Dealing with Aberrant Termination
Use of Stop Codons to Insert Unusual Amino Acids
18.5 Posttranslation
Folding Nascent Proteins
Release of Ribosomes from mRNA
CHAPTER 19 Ribosomes and Transfer RNA
19.1 Ribosomes
Fine Structure of the 70S Ribosome
Ribosome Composition
Fine Structure of the 30S Subunit
Fine Structure of the 50S Subunit
Ribosome Structure and the Mechanism of Translation
Polysomes
19.2 Transfer RNA
The Discovery of tRNA
tRNA Structure
Recognition of tRNAs by Aminoacyl-tRNA Synthetase: The Second Genetic Code
Proofreading and Editing by Aminoacyl-tRNA Synthetases
PART VII DNA Replication, Recombination, and Transposition
CHAPTER 20 DNA Replication, Damage, and Repair
20.1 General Features of DNA Replication
Semiconservative Replication
At Least Semidiscontinuous Replication
Priming of DNA Synthesis
Bidirectional Replication
Rolling Circle Replication
20.2 Enzymology of DNA Replication
Three DNA Polymerases in E. coli
Fidelity of Replication
Multiple Eukaryotic DNA Polymerases
Strand Separation
Single-Strand DNA-Binding Proteins
Topoisomerases
20.3 DNA Damage and Repair
Damage Caused by Alkylation of Bases
Damage Caused by Ultraviolet Radiation
Damage Caused by Gamma and X-Rays
Directly Undoing DNA Damage
Excision Repair
Double-Strand Break Repair in Eukaryotes
Mismatch Repair
Failure of Mismatch Repair in Humans
Coping with DNA Damage Without Repairing It
CHAPTER 21 DNA Replication II: Detailed Mechanism
21.1 Initiation
Priming in E. coli
Priming in Eukaryotes
21.2 Elongation
Speed of Replication
The Pol III Holoenzyme and Processivity of Replication
21.3 Termination
Decatenation: Disentangling Daughter DNAs
Termination in Eukaryotes
Box 21.1 Telomeres, the Hayflick Limit, and Cancer
Telomere Structure and Telomere-Binding Proteins in Lower Eukaryotes
CHAPTER 22 Homologous Recombination
22.1 The RecBCD Pathway for Homologous Recombination
22.2 Experimental Support for the RecBCD Pathway
RecA
RecBCD
RuvA and RuvB
RuvC
22.3 Meiotic Recombination
The Mechanism of Meiotic Recombination: Overview
The Double-Stranded DNA Break
Creation of Single-Stranded Ends at DSBs
22.4 Gene Conversion
CHAPTER 23 Transposition
23.1 Bacterial Transposons
Discovery of Bacterial Transposons
Insertion Sequences: The Simplest Bacterial Transposons
More Complex Transposons
Mechanisms of Transposition
23.2 Eukaryotic Transposons
The First Examples of Transposable Elements: Ds and Ac of Maize
P Elements
23.3 Rearrangement of Immunoglobulin Genes
Recombination Signals
The Recombinase
Mechanism of V(D)J Recombination
23.4 Retrotransposons
Retroviruses
Retrotransposons
PART VIII Genomes
CHAPTER 24 Introduction to Genomics: DNA Sequencing on a Genomic Scale
24.1 Positional Cloning: An Introduction to Genomics
Classical Tools of Positional Cloning
Identifying the Gene Mutated in a Human Disease
24.2 Techniques in Genomic Sequencing
The Human Genome Project
Vectors for Large-Scale Genome Projects
The Clone-by-Clone Strategy
Shotgun Sequencing
Sequencing Standards
24.3 Studying and Comparing Genomic Sequences
The Human Genome
Personal Genomics
Other Vertebrate Genomes
The Minimal Genome
The Barcode of Life
CHAPTER 25 Genomics II: Functional Genomics, Proteomics, and Bioinformatics
25.1 Functional Genomics: Gene Expression on a Genomic Scale
Transcriptomics
Genomic Functional Profiling
Single-Nucleotide Polymorphisms: Pharmacogenomics
25.2 Proteomics
Protein Separations
Protein Analysis
Quantitative Proteomics
Protein Interactions
25.3 Bioinformatics
Finding Regulatory Motifs in Mammalian Genomes
Using the Databases Yourself
Glossary
Index